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Approval status
BiomarkerPrevalenceTests / detection methodTumour types
ALK gene rearrangements occur in approximately 3%-7% of NSCLC, more common in younger patients and never-smokers.[fda]FISH, IHC, NGS (fusion calling)non-small cell lung cancer
BRAF V600E mutations occur in approximately 50% of cutaneous melanomas, 8%-15% of colorectal cancers, and 1%-3% of NSCLC.[fda]PCR-based mutation assays, NGScolorectal cancer, melanoma, non-small cell lung cancer
Pathogenic germline BRCA1 variants are found in approximately 5% of unselected breast cancers and up to 15% of ovarian cancers.[fda]germline genetic testing, somatic NGSbreast cancer, ovarian cancer, pancreatic cancer, prostate cancer
Pathogenic germline BRCA2 variants are found in approximately 5% of unselected breast cancers and are also associated with elevated risk of ovarian, pancreatic, and prostate cancer.[fda]germline genetic testing, somatic NGSbreast cancer, ovarian cancer, pancreatic cancer, prostate cancer
Activating EGFR mutations occur in approximately 10%-15% of NSCLC in Western populations and approximately 30%-40% in Asian and never-smoker populations.[fda]NGS, PCR-based mutation panelsnon-small cell lung cancer
HER2 is overexpressed or amplified in approximately 15%-20% of breast cancers and approximately 15%-20% of gastric/gastroesophageal junction adenocarcinomas.[fda]IHC, ISH/FISH, NGS (amplification calling)breast cancer, gastric cancer, gastroesophageal junction adenocarcinoma
KRAS mutations occur in approximately 25% of NSCLC and up to 45% of colorectal cancers; KRAS G12C specifically accounts for approximately 13% of NSCLC and 3%-4% of colorectal cancer cases.[fda]NGScolorectal cancer, non-small cell lung cancer, pancreatic cancer
MET exon 14 skipping alterations occur in approximately 3%-4% of NSCLC, more common in older patients and those with sarcomatoid histology.[fda]NGS (RNA-based assays preferred for splice variants)non-small cell lung cancer
PD-L1 expression thresholds and prevalence vary substantially by tumor type and assay; high PD-L1 expression (TPS >= 50%) is seen in approximately 25%-30% of NSCLC by validated companion diagnostic assays.[fda]IHCgastric cancer, non-small cell lung cancer, triple-negative breast cancer, urothelial carcinoma
PIK3CA hotspot mutations are found in approximately 40% of hormone receptor-positive, HER2-negative advanced breast cancers.[fda]NGS, PCR-based mutation assaysbreast cancer